Journal: Frontiers in Immunology

Article Title: Hyaluronic acid−CD44 signaling from decidual stromal cells orchestrates dNK1 differentiation and immune tolerance in early pregnancy

doi: 10.3389/fimmu.2026.1777567

Figure Lengend Snippet: HA-induced activation of the canonical Wnt pathway upregulates FOSL2 to expand the dNK1-like subpopulation. (A) Analysis of transcriptional activity and expression levels of transcription factors in three dNK subsets using scRNA-seq data from normal decidual tissue ( n = 11). (B) Transcriptional activity and expression levels of FOSL2 in decidual tissues from normal pregnancy ( n = 5) and RSA groups ( n = 3). (C) Changes in NK92MI cells of the five transcription factors ( STAT3, MAFB, HES1, FOSL2, ETV5 ) characterized by high transcriptional activity and expression in the dNK1 subset following HMW-HA treatment, as determined by RT−qPCR ( n = 6 per group). (D) FOSL2 expression in NK92MI cells following HMW-HA treatment, assessed by WB ( n = 3 per group). (E) Wnt1 and β-catenin protein expression in NK92MI cells treated with or without HMW-HA in the presence or absence of CD44 blockade ( n = 3 per group). (F) FOSL2 expression in HA−treated NK92MI cells after addition of CD44−blocking antibody or the Wnt pathway inhibitor IWP−2 to the culture system ( n = 3 per group). Data are expressed as mean ± SD; * P < 0.05; ** P < 0.01; ns, not significant. EP, early pregnancy; SA, spontaneous abortion; RSA, recurrent spontaneous abortion.

Article Snippet: The PVDF membranes were incubated overnight at 4 °C with primary antibodies against HAS2 (1:1000; 63kD; Santa Cruz, sc-514737), FOSL2 (1:1000; 45kD; Proteintech, M02615-2), Wnt1 (1:500; 45kD; Boster, PB0408), β-catenin (1:500; 92kD; CST, 8480T) and GAPDH (1:5000; 36kD; Proteintech, HRP-60004).

Techniques: Activation Assay, Activity Assay, Expressing, Quantitative RT-PCR, Blocking Assay

Journal: Molecular Medicine

Article Title: Wnt5a-mediated autophagy contributes to the epithelial-mesenchymal transition of human bronchial epithelial cells during asthma

doi: 10.1186/s10020-024-00862-3

Figure Lengend Snippet: Schematic illustration of the mechanisms of Wnt5a-induced EMT in HBECs. This hypothesis illustrates a molecular pathway in HBECs leading to airway remodeling, typically associated with classical autophagy pathway and EMT. In short, Wnt5a induced by HDM or IL-4 leads to the overactivation of autophagy in HBECs through the Ca 2+ /CaMKII signaling pathway, subsequently promoting bronchial epithelial EMT and triggering airway remodeling

Article Snippet: The antibodies used in the present research were as follows: rabbit anti-α-Tubulin antibody (1:2000, Servicebio, Wuhan, China); rabbit anti-α-SMA antibody (1:1000, Cell Signaling Technology, USA); rabbit anti-E-cadherin antibody (1:2000, Cell Signaling Technology); rabbit anti-Beclin1 antibody (1:1500, Cell Signaling Technology); rabbit anti-Collagen I antibody (1:1000, Cell Signaling Technology); rabbit anti-LC3 antibody (1:1500, Cell Signaling Technology); rabbit anti-Wnt5a antibody (1:1000, BOSTER, Wuhan, China); rabbit anti-CaMKII antibody (1:1000, BOSTER); rabbit anti-p-CaMKII antibody (1:1000, Cell Signaling Technology).

Techniques:

Journal: American Journal of Translational Research

Article Title: Osteoblast-activating peptide exhibits a specific distribution pattern in mouse ovary and may regulate ovarian steroids and local calcium levels

doi:

Figure Lengend Snippet: List of antibodies, sources, working dilutions, and methods of antigen retrieval

Article Snippet: Rabbit monoclonal anti-calmodulin , (BS9898M, Bioworld Technology, Minneapolis, Minnesota, USA) , 1:300 , 10 mM citrate buffer (pH 6.0) , 105°C, 20 min , --.

Techniques:

Journal: American Journal of Translational Research

Article Title: Osteoblast-activating peptide exhibits a specific distribution pattern in mouse ovary and may regulate ovarian steroids and local calcium levels

doi:

Figure Lengend Snippet: Immunofluorescence localization of estrogen receptor α (ER-α) (red; A, D, and G) and calmodulin (green; B, E, and H) in the mouse ovary. In (A-C), the corpus luteum reveals a moderately positive reaction for ER-α and an intensely positive reaction for calmodulin (asterisks). The immature corpus luteum shows positivity for ER-α and negativity for calmodulin (bent line). In (D-F), granulosa cells of tertiary follicle reveal an intensely positive reaction for ER-α, but a negative reaction for calmodulin, except at the periphery of the follicle (thick arrows). In (G-I), the reactivity for calmodulin is located between the ER-α-immunopositive cells (arrowheads). Scale bar = 200 μm. (J) The average integrated densities of staining for different parts of the ovary for ER-α. (K) The average integrated densities of staining for different parts of the ovary for calmodulin. Data are expressed as the mean ± SE. Bars with different superscripts are significantly different (Duncan’s new multiple range test was used to compare the means among treatments; P < 0.05). PF, primary follicle; SF, secondary follicle; MGF, mature Graafian follicle; MCL, mature corpus luteum; ICL, immature corpus luteum; IC, interstitial cell.

Article Snippet: Rabbit monoclonal anti-calmodulin , (BS9898M, Bioworld Technology, Minneapolis, Minnesota, USA) , 1:300 , 10 mM citrate buffer (pH 6.0) , 105°C, 20 min , --.

Techniques: Immunofluorescence, Staining

Journal: American Journal of Translational Research

Article Title: Osteoblast-activating peptide exhibits a specific distribution pattern in mouse ovary and may regulate ovarian steroids and local calcium levels

doi:

Figure Lengend Snippet: Correlation coefficient (r) between the examined parameters

Article Snippet: Rabbit monoclonal anti-calmodulin , (BS9898M, Bioworld Technology, Minneapolis, Minnesota, USA) , 1:300 , 10 mM citrate buffer (pH 6.0) , 105°C, 20 min , --.

Techniques: